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產品名稱：重組大鼠單核細胞趨化蛋白3 / CCL7蛋白
產品型號：rRtMCP-3/CCL7
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠單核細胞趨化蛋白3 / CCL7蛋白與其它公司提供的重組蛋白不同，rRtMCP-3/CCL7蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠單核細胞趨化蛋白3 / CCL7蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠單核細胞趨化蛋白3 / CCL7蛋白

Synonyms Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC

Species Rat

Accession Q9QXY8

GeneID 287561

Source 重組大鼠單核細胞趨化蛋白3 / CCL7蛋白Escherichia coli.

Molecular Weight Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP

Purity 重組大鼠單核細胞趨化蛋白3 / CCL7蛋白> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重組大鼠單核細胞趨化蛋白3 / CCL7蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRtMCP-3/CCL7 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠單核細胞趨化蛋白3 / CCL7蛋白

SDS-PAGE

Reference 1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8.
2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9.

Background Rat CCL7, belonging to the CC chemokine family, is also named as MCP-3, because the sequence comparison suggested that the rat CCL7 is a homologue of the human MCP-3 and is postulated the rat MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic functions for monocytes and eosinophils, but not for neutrophils. Additionally, it can activated NK cells as well as CD4+ and CD8+ T lymphocytes. CCL7 was found to interact with MMP2.

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Synonyms	Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC
Species	Rat
Accession	Q9QXY8
GeneID	287561
Source	重組大鼠單核細胞趨化蛋白3 / CCL7蛋白Escherichia coli.
Molecular Weight	Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP
Purity	重組大鼠單核細胞趨化蛋白3 / CCL7蛋白> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重組大鼠單核細胞趨化蛋白3 / CCL7蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rRtMCP-3/CCL7 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠單核細胞趨化蛋白3 / CCL7蛋白
SDS-PAGE
Reference	1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8. 2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9.
Background	Rat CCL7, belonging to the CC chemokine family, is also named as MCP-3, because the sequence comparison suggested that the rat CCL7 is a homologue of the human MCP-3 and is postulated the rat MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic functions for monocytes and eosinophils, but not for neutrophils. Additionally, it can activated NK cells as well as CD4+ and CD8+ T lymphocytes. CCL7 was found to interact with MMP2.

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產品名稱：重組大鼠單核細胞趨化蛋白3 / CCL7蛋白